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KMID : 1204720080010010082
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International Journal of Stem Cells
2008 Volume.1 No. 1 p.82 ~ p.90
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Induction of Nestin-expressing Spheroids from Human Dermal Fibroblasts in a bFGF-dependent Manner
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Son Young-Sook
Lee Dong-Sun
Lee Ji-Yeon
Chi Guangfan
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Abstract
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Background and Objectives: Neural stem/precursor cells are found in relatively inaccessible neurogenic regions of the adult brain, making them difficult to harvest for therapeutic purposes.
Methods and Results: In this study, nestin-expressing spheroids were induced from primary cultures of newborn and adult foreskin-derived dermal fibroblasts using a novel, two-step induction method. Approximately 80% of dermal fibroblasts became nestin-expressing cells within 12 days. Nestin expression and spheroid-forming capacity were both blocked by removal of bFGF from the induction medium. The bFGF-induced, nestin-expressing spheroids possessed most of the features distinctive of skin-derived precursor cells.
Conclusions: Our findings support the possibility of inducing nestin-expressing spheroids from purified human dermal fibroblasts in a bFGF dependent manner. This is important because it may be impossible to induce spheroids from the small pool of residual neural crest-related precursor cells found in adults.
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KEYWORD
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skin-derived precursors, dermal fibroblast, nestin, spheroids, neural crest stem cells, cell transdifferentiation
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