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KMID : 1204720090020010045
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International Journal of Stem Cells
2009 Volume.2 No. 1 p.45 ~ p.50
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In Vitro Differentiation and Expansion of Intrathymic T Cell Progenitors from Human Umbilical Cord Blood-Derived CD34＋ Cells
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Sun Bo
Park Sang-Bum
Jung Ji-Won
Seo Kwang-Won
Lee Yong-Soon
Kang Kyung-Sun
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Abstract
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Background and Objectives: CD4 positive cells play a central role in many lethal diseases, such as AIDS, cancer and autoimmunity diseases. CD4?commitment of hematopoietic stem cells involved in T cell lineage, monocyte and dendritic cells development. In this study, we showed that CD4 commitment out of thymus which may happen when hematopoietic cells undergo monocyte , dendritic cells or even earlier T cell progenitor differentiation.
Methods and Results: after culturing in our medium for more than five weeks, CD4?CD34＋fraction, isolated from human umbilical cord blood, decreased to 1%. However, the fraction expressing CD4 went up to 86.5%. After CD4＋cells were cultured in methylcellulose-based CFU medium, about 40 colonies/2×104 cells could developed. An activation of notch-1 pathway in the freshly isolated CD34＋cells and up-regulation of PI3K/JNK/c-Myc pathway may provide an explanation for the differentiation and proliferation of CD4＋ cells from CD34＋ hematopoieticstemcellsrespectively.
Conclusions: ACD4＋ enrichedpopulationwasobtainedafterhighlypurified CD34＋ cells, isolated from human cord blood, underwent long term culture in a feeder layer-free culturing system. Colonigenic ability was maintained in the population of CD4＋ cells. This finding will be a benefit for the studies on the cell therapy for immune dysfunctions.
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KEYWORD
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CD34＋ cells, Differentiation, Intrathymic T cell progenitors, Propagation, Umbilical cord blood
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