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KMID : 1204720090020020129
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International Journal of Stem Cells
2009 Volume.2 No. 2 p.129 ~ p.134
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The Primary Study of CD90＋CD34?and Sca-1＋ Stem Cells Mobilized by EPO Plus G-CSF in Mice
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Zheng Fang
Cheng Long
Yu Qiang
Liu Qihuan
Cheng Fanjun
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Abstract
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To investigate effects of recombinant human erythropoietin（rhEPO） plus recombinant human granulocyte colony stimulating factor（rhG-CSF）on mobilization of CD90＋CD34? and Sca-1＋ stem cells in mice. rhEPO 1000 IU·kg?1ㆍd?1 per mice alone or plus rhG-CSF 250 ?g·kg?1ㆍd-1?1per mice was administered to mice for five days，the peripheral blood was collected at 6 hours, 1, 3,5 and 7 days after the last administration. The number of white blood cells (WBC) and mononuclear cells (MNC) was counted. The level of CD34, CD90 and Sca-1 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR).The express- ions of CD90＋CD34? in absolute nuclear cells of peripheral blood was detected by flow cytometry. The results indicate that compared to mobilizing by rhG-CSF, rhEPO or plus rhG-CSF did not significantly enhance the number of WBC and MNC, on the fifth and the seventh day after the last administration, both the expression of CD34,CD90 and Sca-1 mRNA and the proportion of CD90＋CD34? cells dramatically raised in rhEPO or in addition to rhG-CSF groups. We conclude that rhEPO or plus rhG-CSF had a strong capacity of mobilization of stem cells. The alteration of expression of CD34, CD90 and Sca-1 mRNA after the administration of rhEPO or combined with rhG-CSF indicated that this agents might potentially alter the peripheral blood graft content.
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KEYWORD
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Erythropoietin, Granulocyte-colony stimulating factor, Mobilization, Peripheral blood, Stem cells
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