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KMID : 1204720090020020135
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International Journal of Stem Cells
2009 Volume.2 No. 2 p.135 ~ p.140
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Adipose Tissue is A Better Source of Immature Non-Hematopoietic Cells than Bone Marrow
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Fujimura Juri
Sugihara Hidemitsu
Fukunaga Yoshitaka
Suzuki Hidenori
Ogawa Rei
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Abstract
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Adipose tissue (AT) is an alternative source of the adult stem cells that can also be harvested from bone marrow (BM). Cultured AT-derived stem cells (ASCs) have been well characterized by many groups. However, non-cultured ASCs remain to be characterized. Hoechst 33342 dye efflux is a characteristic that is common to stem cells, as well as chemotherapy-resistant cancer cells. Thus, we compared the Hoechst 33342-stained side population (SP) cells in murine adipose-tissue (AT-SP cells) to the SP cells from murine bone marrow (BM-SP cells). The AT-SP cells were detected much more frequently in the 22 AT samples that were tested (0.42∼6.00%, mean 2.57%) than the BM-SP cells were detected in the 6 BM samples (0.02∼0.36%, mean 0.12%). After Hoechst staining, SP cells were analyzed by fluorescence-activated cell sorting (FACS) and electron micrograms. FACS analysis revealed that the AT-SP cells were CD44-, CD45-, CD45R＋, Sca-1± and c-kit-, while the BM-SP cells were CD44-, CD45±, CD45R-, Sca-1＋ and c-kit＋. This indicates that the AT-SP cells differ phenotypically from the BM-SP cells. Electron microscopic analysis revealed that the AT-SP cells are small cells with a diameter of about 5 um. Some of the BM-SP cells had granules, similar to eosinophils or basophils, whereas the AT-SP cells had fewer organelles and a higher N/C ratio than the BM-SP cells. This suggests that the AT-SP cells are considerably more immature than the BM-SP cells. Thus, it appears that AT is a better source of immature non-hematopoietic cells than BM.
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KEYWORD
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Adipose-derived stem cells, Adipose stem cells, Mesenchymal stem cells, Adipose tissue, Sp cells, Bone marrow
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