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KMID : 1204720100030020069
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International Journal of Stem Cells
2010 Volume.3 No. 2 p.69 ~ p.73
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Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering
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Jiang Hu-Lin
Cho Ki-Hyun
Jang Young-Chul
Choi Yun-Jaie
Chung Jong-Hoon
Cho Chong-Su
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Abstract
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The liver plays an important role in a broad spectrum of physiological functions and detoxifies endogenous and exogenous substances. The liver failure is associated with a high risk of mortality because it is one of important organs in our body. Various bioartificial liver (BAL) systems have been used for clinical trials as a bridge for liver transplantations in patients with liver failure. Long term and stable liver-specific functions of hepatocytes in the development of BAL support systems should be considered. Spheroid formation of hepatocytes enhances liver-specific functions. In this review, hepatocyte spheroid formation methods such as galactose density, topology of extracellular matrix, micromolding technique, hanging-drop culture, non-adhesive surface, positive charged surface, spinner culture, rocked technique, medium component, external forces, coculture system and polymeric nanospheres are explained for enhancing liver-specific functions.
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KEYWORD
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Spheroid, Hepatocyte, Liver function, Tissue engineering, Extracellular matrix
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