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KMID : 1204720110040020099
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International Journal of Stem Cells
2011 Volume.4 No. 2 p.99 ~ p.104
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Isolation and Morphological Characterisation of Ovine Adipose-Derived Mesenchymal Stem Cells in Culture
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Grzesiak Jakub
Marycz Krzysztof
Wrzeszcz Karol
Czogala Joanna
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Abstract
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Background and Objectives: AD-MSCs (adipose-derived mesenchymal stem cells), as an easy obtainable population of multipotent cells, have been successfully applied in many diseases in animals. Having very similar properties and morphology to these collected from bone marrow, are very attractive object for regenerative medicine.
Methods and Results: Ovine stem cells applications were considered to serve as suitable animal model for large animals locomotive system disorders therapy. In this research, sheep fat was collected from four different regions of the body. AD-MSC were isolated and cultured for morphological, morphometrical and immunophenotypical analysis. On the basis of conducted study, cells isolated from topographically different part of the body of given animal were almost identical. Morphologically and immunologically, they are very similar to other species. Moreover, application of light and electron microscopy, as well as immunolabeling and measurement assays allowed for detail morphological and biofunctional analysis of ovine adipose-derived mesenchymal stem cells in the culture.
Conclusions: Ovine subcutaneous fat from many regions of the body is rich source of stem cells, which could be used in locomotive system disorders experimental therapy on sheep models.
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KEYWORD
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AD-MSC, Ovis, Immunophenotype, Morphometry, SEM
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