|
KMID : 1204720180110020187
|
|
International Journal of Stem Cells
2018 Volume.11 No. 2 p.187 ~ p.195
|
|
|
MiR-9 Controls Chemotactic Activity of Cord Blood CD34＋ Cells by Repressing CXCR4 Expression
|
|
Ha Tae-Won
Kang Hyun-Soo
Kim Tae-Hee
Kwon Ji-Hyun
Kim Hyun-Kyu
Ryu Ae-Li
Jeon Hye-Ji
Han Jae-Seok
Broxmeyer Hal E.
Hwang Yong-Sung
Lee Yun-Kyung
Lee Man-Ryul
|
|
|
Abstract
|
|
|
|
Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34＋ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34＋ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34＋ cells.
|
|
|
KEYWORD
|
|
|
microRNA, CXCR4, Cord blood, Cell migration
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|