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KMID : 1204720200130030404
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International Journal of Stem Cells
2020 Volume.13 No. 3 p.404 ~ p.413
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Upregulation of C-Reactive Protein by Placenta-Derived Mesenchymal Stem Cells Promotes Angiogenesis in A Rat Model with Cirrhotic Liver
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Jun Ji-Hye
Jung Ji-Eun
Kim Jae-Yeon
Hwang Seong-Gyu
Bae Si-Hyun
Kim Gi-Jin
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Abstract
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Background and Objectives: Liver cirrhosis is accompanied by abnormal vascular shunts. The Wnt pathway is essential for endothelial cell survival and proliferation. C-reactive protein (CRP), which is produced by hepatocyte, activates angiogenesis in cardiovascular diseases.
Methods and Results: The expression of CRP in CCl4-injured rat livers was detected using qRT-PCR and Western blotting after transplantation of placenta-derived mesenchymal stem cells (PD-MSCs) into rats. To determine whether CRP functions in hepatic regeneration by promoting angiogenesis through the Wnt pathway, we detected VEGF and β-catenin in liver tissues and BrdU and β-catenin in hepatocytes by immunofluorescence. The expression levels of CRP, Wnt pathway-related and angiogenic factors were increased in CCl4-injured and PD-MSCs transplanted rat livers. In vitro, the expression levels of Wnt signaling and angiogenic factors were decreased in siRNA-CRP-transfected rat hepatocytes.
Conclusions: CRP upregulation by PD-MSCs participates in vascular remodeling to promote liver regeneration via the Wnt signaling pathway during hepatic failure.
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KEYWORD
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Liver cirrhosis, C-reactive protein, Placenta-derived mesenchymal stem cells, Wnt pathway, Vascular remodeling
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