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KMID : 1204720220150030258
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International Journal of Stem Cells
2022 Volume.15 No. 3 p.258 ~ p.269
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Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS)
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Kang Kyu-Ree
Kim C-Yoon
Kim Jin
Ryu Bo-Kyeong
Lee Seul-Gi
Baek Ji-Eun
Kim Ye-Ji
Lee Jin-Moo
Lee Yoot-Mo
Choi Sun-Ok
Woo Dong-Ho
Park Il-Hwan
Chung Hyung-Min
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Abstract
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Background and Objectives: Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.
Methods and Results: In our study, we developed an in vitro neurotoxicity assessment focusing on neuronal function. To minimize the differences between species and fast screening, hiPSC-derived neurons and a microelectrode array (MEA) that could simultaneously measure the action potentials of the neuronal networks were used. After analyzing the molecular and electrophysiological characters of our neuronal network, we conducted a neurotoxicity assessment on neurotransmitters, neurotoxicants, illicit drugs, and new psychoactive substances (NPS). We found that most substances used in our experiments responded more sensitively to our MEA-based neurotoxicity assessment than to the conventional neurotoxicity assessment. Also, this is the first paper that evaluates various illicit drugs and NPS using MEA-based neurotoxicity assessment using hiPSC-derived neurons.
Conclusions: Our study expanded the scope of application of neurotoxicity assessment using hiPSC-derived neurons to NPS, and accumulated evaluation data of various toxic substances for hiPSC-derived neurons.
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KEYWORD
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Neurotoxicity assessment, Microelectrode array, iPSC-derived neuron application, Illicit drugs, New psychoactive substance
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