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KMID : 1236020190470010011
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.11 ~ p.19
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Characterization of the Growth, Total Lipid and Fatty Acid Profiles in Microalga, Nannochloropsis oceanica under Different Nitrogen Sources
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Mahdieh Majid
Shabani Salimeh
Amirjani Mohammad Reza
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Abstract
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The properties of microalgae as bioresources for biodiesel production can be improved by adding nitrogen sources into the culture medium. Thus, Nannochloropsis oceanica CCAP 849/10 was cultured in f/2 media supplemented with five different forms of nitrogen at 0.88 mmol-N l-1 each: ammonium bicarbonate (NH4HCO3), ammonium sulfate ((NH4)2SO4), sodium nitrate (NaNO3), ammonium nitrate (NH4NO3), and urea. The cell density, lipid content, and fatty acid profile of the microalga were determined after 15 days of cultivation. The growth of N. oceanica based on cell number was lowest in the medium with NH4NO3, and increased significantly in the medium with NH4HCO3. Cells treated with (NH4)2SO4, and NH4NO3 produced the highest total lipid contents (i.e., 65% and 62% by dry weight, respectively). The fatty acid profiles of the microalga were significantly different in the various nitrogen sources. The major fatty acids detected in cultures supplemented with NH4HCO3, (NH4)2SO4, NH4NO3, or urea were C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C20:5, and C22:6. However, the C16:1 content in the NaNO3-supplemented culture was very low. This study highlights that the nitrogen source can strongly influence lipid production in N. oceanica and its fatty acid composition.
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KEYWORD
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Biodiesel, Chlorophyll a, nutrient, microalga, urea
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