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KMID : 1236020190470010078
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.78 ~ p.86
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Metabolic Engineering of Corynebacterium glutamicum for N-acetylglucosamine Production
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Kim Jin-Yeon
Kim Bu-Yeon
Moon Kyung-Ho
Lee Jin-Ho
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Abstract
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Recombinant Corynebacterium glutamicum producing N-acetylglucosamine (GlcNAc) was constructed by metabolic engineering. To construct a basal strain producing GlcNAc, the genes nagA, nagB, and nanE encoding N-acetylglucosamine-6-phosphate deacetylase, glucosamine-6-phosphate deaminase, and Nacetylmannosamine- 6-phosphate epimerase, respectively, were sequentially deleted from C. glutamicum ATCC 13032, yielding strain KG208. In addition, the genes glmS and gna1 encoding glucosamine-6-phosphate synthase and glucosamine-6-phosphate N-acetyltransferase, which originated from C. glutamicum and Saccharomyces cerevisiae, respectively, were expressed in several expression vectors. Among several combinations of glmS and gna1 expression, recombinant cells expressing glmS and gna1 under control of the ilvC promoter produced 1.77 g/l of GlcNAc and 0.63 g/l of glucosamine in flask cultures.
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KEYWORD
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N-acetylglucosamine, glucosamine, Corynebacterium glutamicum, metabolic engineering
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