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KMID : 1236020190470010096
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.96 ~ p.104
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Kinetics of Enriched Chitinase as Extracellular Metabolite in Beauveria bassiana
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Mondal Subhoshmita
Datta Siddhartha
Mukherjee Alakananda
Bhattacharya Pinaki
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Abstract
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Beauveria bassiana one of the most common entomopathogenic fungi has been isolated, pre defined and characterized in-house from soil of tea cultivation area. Experiments have been performed to verify the presence of chitinase as intracellular metabolite and its release as extracellular product rendering the spores with biopesticide activity. Although there are many responsible enzymes for the pest killer action of Beauveria bassiana, binding property of chitinase depending on presence as well as absence of serine supplemented in the media has been studied with respect to the production and kinetics. A programmed investigation conclusively indicates that the isolated spore (hyphae) Beauveria bassiana has been metabolically enriched with the enzyme chitinase in presence of an externally added amino acid serine with its inhibitory kinetics.
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KEYWORD
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Beauveria bassiana, serine, chitinase, inhibitory kinetics, biopesticide activity
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