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KMID : 1236020190470010158
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 1 p.158 ~ p.163
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A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus
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Khodabakhsh Tannaz
Arabi Azin
Pakzad Parviz
Gheflat Shiva Sadat
Bahreinipour Akram
Bandehpour Mojgan
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Abstract
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Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.
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KEYWORD
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Brucella abortus, protein expression, antigenic epitopes, VirB2.3.9, ELISA
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