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KMID : 1236020190470040530
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 4 p.530 ~ p.535
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Isolation of Xylitol-Producing Thermotolerant Yeast Millerozyma farinosa from Nuruk
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Jung Eun-Hye
Bae Young-Woo
Kwun Se-Young
Park Eun-Hee
Kim Myung-Dong
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Abstract
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Diverse types of nuruks (traditional Korean fermentation initiators) were examined in order to isolate thermotolerant yeast strains capable of utilizing xylose as a carbon source. Among twenty yeast strains that grew at 46℃, MBY/L1597 showed a notably higher specific growth rate than other strains. This strain was identified as Millerozyma farinosa. While the control strain M. farinosa KCTC27412 (= CBS7064) did not show xylose reductase (XR) activity and apparent growth at 46℃, M. farinosa MBY/L1597 exhibited XR activity of 4.98 ± 0.49 U/mg protein when NADPH was used as a cofactor. M. farinosa MBY/L1597 cultured at 46℃ produced (9.87 ± 1.00 g/l) xylitol from 20 g/l xylose, corresponding to approximately 50% yield. M. farinosa MBY/L1597 was deposited at the Korean Collection for Type Cultures as KCTC27797.
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KEYWORD
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Tolerance, xylose reductase, xylitol, yeast, Millerozyma farinosa, Nuruk
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