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KMID : 1236020190470040565
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Microbiology and Biotechnology Letters
2019 Volume.47 No. 4 p.565 ~ p.573
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Development of Pichia stipitis Co-fermenting Cellobiose and Xylose Through Adaptive Evolution
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Kim Dae-Hwan
Lee Won-Heong
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Abstract
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Production of biofuels and value-added materials from cellulosic biomass requires the development of a microbial strain capable of efficiently fermenting mixed sugars. In this study, the natural xylose fermenting yeast, Pichia stipitis, was evolved to simultaneously ferment cellobiose and xylose. Serial subcultures of wild-type P. stipitis in 20 g/l cellobiose were performed to increase the rate of cellobiose consumption. A total of ten rounds of the serial subculture led to the isolation of an evolved strain fermenting cellobiose significantly faster than the parental strain. The evolved strain displayed enhanced ethanol yield from 0 to 0.4 g ethanol/g cellobiose. The evolved P. stipitis simultaneously fermented cellobiose and xylose in batch fermentation. The genetic information of our evolved P. stipitis would be valuable in the development of a microbial host for the production of biofuels and biomaterials from cellulosic biomass.
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KEYWORD
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Pichia stipitis, cellobiose, xylose, co-fermentation, adaptive evolution
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