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KMID : 1237720100430020150
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Anatomy & Cell Biology
2010 Volume.43 No. 2 p.150 ~ p.156
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Phosphorylation of 14-3-3ζ at serine 58 and neurodegeneration following kainic acid-induced excitotoxicity
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Jeong Eun-Ae
Jeon Byeong-Tak
Kim Jeong-Bin
Kim Joon-Soo
Cho Yong-Woon
Lee Dong-Hoon
Kim Hyun-Joon
Kang Sang-Soo
Cho Gyeong-Jae
Choi Wan-Sung
Roh Gu-Seob
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Abstract
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Oxidative stress-induced cell death leads to phosphorylation of 14-3-3ζ at serine 58. 14-3-3ζ is detected at significant levels in cerebrospinal fluid after kainic acid (KA)-induced seizures. Here we examined temporal changes in 14-3-3ζ phosphorylation in the hippocampus and amygdala of mice after KA treatment. Mice were killed at 2, 6, 24, or 48 h after KA (30 mg/kg) injection. We observed an increase in TUNEL and Fluoro-Jade B (FJB)-stained neurons in the hippocampus and amygdala of KA-treated mice. Phospho (p)-14-3-3ζ and p-JNK expression was increased in the hippocampus 2 and 6 h after KA treatment, respectively. In immunohistochemical analysis, p-14-3-3ζ-positive cells were present in the CA3 region of the hippocampus and the central nucleus of amygdala (CeA) of KA-treated mice. Thus, phosphorylation of 14-3-3ζ at serine 58 may play an important role in KA-induced hippocampal and amygdaloid neuronal damage.
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KEYWORD
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Kainic acid, 14-3-3ζ, hippocampus, amygdala, neurodegeneration
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