|
KMID : 4412620180240010005
|
|
Clinical Hypertension
2018 Volume.24 No. 1 p.5 ~ p.5
|
|
|
Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens
|
|
Youn Jong-Chan
Kim Jun-Yong
Jung Min-Kyung
Yu Hee-Tae
Park Su-Hyung
Kim In-Cheol
Lee Sun-Ki
Choi Suk-Won
Han Seong-Woo
Ryu Kyu-Hyung
Park Sung-Ha
Shin Eui-Cheol
|
|
|
Abstract
|
|
|
Background: Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension.
Methods: To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays.
Results: In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes
Conclusions: CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.
|
|
|
KEYWORD
|
|
|
Cytomegalovirus, T cell, Arterial stiffness, Enzyme-linked immunospot (ELISPOT) assay, Intracellular cytokine staining (ICS)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|